Separation of murine peritoneal macrophages using percoll density gradients

Macrophages harvested from the murine peritoneal cavity are functionally and morphologically heterogeneous. Here, we describe a procedure which permits the determination of specific cell densities using a continuous density gradient of Percoll (analytical step). Subsequently, discontinuous density gradients are used in routine (preparative step) to isolate all the cell subpopulations according to their actual specific density. This procedure has been successfully used for both mouse and rat peritoneal macrophages.