In my (DNA) dreams: A tri-G-quadruplex was constructed from three strands (T1–T3) of DNA using duplex formation to guide the G-rich tracts into close proximity with the addition of Li+ ions (see scheme). The defined G-quadruplex structure was formed upon addition of Na+ ions and characterized by gel electrophoresis and spectroscopy. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.