化学
吸附
牛血清白蛋白
聚丙烯酰胺
单体
分子印迹聚合物
丙烯酰胺
分子印迹
聚合
色谱法
高分子化学
水溶液
聚丙烯
自愈水凝胶
化学工程
核化学
聚合物
选择性
生物化学
有机化学
工程类
催化作用
作者
Kongyin Zhao,Beibei Lin,Wenkui Cui,Lingzhi Feng,Chen Tian,Junfu Wei
出处
期刊:Talanta
[Elsevier]
日期:2014-04-01
卷期号:121: 256-262
被引量:67
标识
DOI:10.1016/j.talanta.2014.01.010
摘要
Bovine serum albumin (BSA) imprinted polypropylene (PP) fiber-grafted polyacrylamide (PAM) hydrogel membrane (PP-g-PAM MIP) was prepared using non-woven PP fiber as matrix, BSA as template molecule, and acrylamide (AM) as functional monomer via UV radiation-reduced polymerization in an aqueous phase. SEM, FT-IR, DSC and TG were used to characterize the PP grafted PAM hydrogel. Influence factors on the adsorption capacity of PP-g-PAM MIP were investigated, such as monomer concentration, cross-linker concentration, template molecule amount and pH values in BSA solution. The adsorption and recognition properties of PP-g-PAM MIP were evaluated and the results showed that the PP-g-PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA as compared with non-imprinted ones. PP-g-PAM MIPs could recognize the template protein using Lys, Ova, BHb, and Glo as control proteins, and the selectivity factor (β) was above 2.0. The imprinting efficiency of PP-g-PAM MIP tended to be stable after three cycles and maintained 76% of the initial value of the imprinting efficiency even after five repetitions, which was more excellent than that of PAM microsphere. The PP-g-PAM MIP is low cost and easy to be prepared, which would show its potential applications in the fields of extracting and testing required proteins from cells or particulate samples.
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