Control of Nanomolar Interaction and In Situ Assembly of Proteins in Four Dimensions by Light

Making light work: Photoactivatable multivalent trisNTA compounds have been developed for the in situ labeling and assembly of His-tagged proteins in time and space (see picture). This small light-tunable lock-and-key system offers the opportunity to trigger nanomolar protein interactions, such as receptor clustering, or biotechnological applications, for example, multiprotein arrays.