稻黄单胞菌
WRKY蛋白质结构域
生物
水稻黄单胞菌。稻瘟
植物抗病性
基因
转基因水稻
遗传学
细胞生物学
突变体
黄单胞菌
RNA干扰
麦格纳波特
格里斯麦格纳波特
转基因
拟南芥
转基因作物
水稻
核糖核酸
作者
Kieu Thi Xuan Vo,Chi-Yeol Kim,Trung Viet Hoang,Sang‐Kyu Lee,Gautam Shirsekar,Young-Su Seo,Sang-won Lee,Guo‐Liang Wang,Jong‐Seong Jeon
标识
DOI:10.3389/fpls.2017.02220
摘要
WRKY proteins play important roles in transcriptional reprogramming in plants in response to various stresses including pathogen attack. In this study, we functionally characterized a rice WRKY gene, OsWRKY67, whose expression is upregulated against pathogen challenges. Activation of OsWRKY67 by T-DNA tagging significantly improved the resistance against two rice pathogens, Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo). Reactive oxygen species (ROS) rapidly accumulated in OsWRKY67 activation mutant lines in response to elicitor treatment, compared with the controls. Overexpression of OsWRKY67 in rice confirmed enhanced disease resistance, but led to a restriction of plant growth in transgenic lines with high levels of OsWRK67 protein. OsWRKY67 RNAi lines significantly reduced resistance to M. oryzae and Xoo isolates tested, and abolished XA21-mediated resistance, implying the possibility of broad-spectrum resistance from OsWRKY67. Transcriptional activity and subcellular localization assays indicated that OsWRKY67 is present in the nucleus where it functions as a transcriptional activator. Quantitative PCR revealed that the pathogenesis-related genes, PR1a, PR1b, PR4, PR10a, and PR10b, are upregulated in OsWRKY67 overexpression lines. Therefore, these results suggest that OsWRKY67 positively regulates basal and XA21-mediated resistance, and is a promising candidate for genetic improvement of disease resistance in rice.
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