糖酵解
磷酸肌酸
肌酸
安普克
内科学
内分泌学
化学
肌酸激酶
三磷酸腺苷
新陈代谢
生物化学
生物
能量代谢
医学
酶
蛋白激酶A
作者
Lin Zhang,Xiaofei Wang,Jiaolong Li,Xudong Zhu,Feng Gao,Guanghong Zhou
标识
DOI:10.1021/acs.jafc.7b02740
摘要
Creatine monohydrate (CMH) contributes to reduce transport-induced muscle rapid glycolysis and improve meat quality of broilers, but the underlying mechanism is still unknown. Therefore, this study aimed to investigate the molecular mechanisms underlying the ameliorative effects of CMH on muscle glycolysis metabolism of transported broilers during summer. The results showed that 3 h transport during summer elevated chicken live weight loss and plasma corticosterone concentration; decreased muscle concentrations of ATP, creatine, and energy charge value; increased muscle AMP concentration and AMP/ATP ratio; and upregulated muscle mRNA expression of LKB1 and AMPKα2, as well as protein expression of p-LKB1Thr189 and p-AMPKαThr172, which subsequently resulted in rapid glycolysis in the pectoralis major muscle and consequent reduction of meat quality. Dietary addition of CMH at 1200 mg/kg ameliorated transport-induced rapid muscle glycolysis and reduction of meat quality via enhancement of the energy-buffering capacity of intramuscular phosphocreatine/creatine system and inhibition of AMPK pathway.
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