De Novo Biosynthesis of 1,5‐Pentanediol by Metabolically Engineered Escherichia coli

, which had enhanced activity against 5-HV and reduced its accumulation. Subsequently, the accumulation of 5-HV was further reduced by enhancing the expression of CAR through RBS engineering and fixing CAR with the help of EutM protein scaffold. In addition, the endogenous gene ycjQ of E. coli was deleted to reduce the reoxidation of 1,5-PDO. Finally, the 1,5-PDO yield reached 12.9 g/L under the optimized fermentation conditions, achieving efficient biosynthesis of 1,5-PDO and lower accumulation of 5-HV, which is the highest yield reported in E. coli so far.