细胞凋亡
胶体金
癌细胞
线粒体
光热治疗
生物物理学
细胞生物学
材料科学
转染
化学
纳米颗粒
癌症
纳米技术
生物化学
生物
基因
遗传学
作者
Msaukiranji Mkandawire,Matthias Lakatos,Armin Springer,Andre Clemens,Dietmar Appelhans,Udo Krause-Buchholz,W. Pompe,Gerhard Rödel,Martin Mkandawire
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2015-01-01
卷期号:7 (24): 10634-10640
被引量:69
摘要
A major challenge in designing cancer therapies is the induction of cancer cell apoptosis, although activation of intrinsic apoptotic pathways by targeting gold nanoparticles to mitochondria is promising. We report an in vitro procedure targeting mitochondria with conjugated gold nanoparticles and investigating effects on apoptosis induction in the human breast cancer cell line Jimt-1. Gold nanoparticles were conjugated to a variant of turbo green fluorescent protein (mitoTGFP) harbouring an amino-terminal mitochondrial localization signal. Au nanoparticle conjugates were further complexed with cationic maltotriose-modified poly(propylene imine) third generation dendrimers. Fluorescence and transmission electron microscopy revealed that Au nanoparticle conjugates were directed to mitochondria upon transfection, causing partial rupture of the outer mitochondrial membrane, triggering cell death. The ability to target Au nanoparticles into mitochondria of breast cancer cells and induce apoptosis reveals an alternative application of Au nanoparticles in photothermal therapy of cancer.
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