Surface Modification of Glass Slides with Aminosilanes for Microarray Use

Glass serves as the solid support for a variety of array types; however, the chemical nature of glass makes it unsuitable for high-affinity binding to most biomolecules. In this chapter, we describe the activation and surface coating of glass with silane, a wide-ranging group of molecules that can covalently attach to the surface of glass and modify it with a variety of functional groups.