Advanced Platelet Rich Fibrin APRF Comparison of Lymphocyte Cellularity as an Age Indicator Between Two Age Groups

The study assesses the cells in platelet-rich fibrin using advanced platelet-rich fibrin and standard platelet-rich fibrin protocol between two age groups. Platelet-rich fibrin (PRF) is a fibrin matrix in which various cells are trapped including platelets and growth factors. It was first developed by Choukron et al. in France in the year 2001, It is a second-generation platelet concentrate developed for use specifically in oral and maxillofacial surgery. It is known to have various regenerative and healing properties including tissue regeneration, revascularization, osteogenic properties, etc. making it ideal for wound healing. PRF can be classified into two types based on the centrifugation protocol as standard–platelet-rich fibrin (S-PRF) and advanced platelet-rich fibrin (A-PRF). This study required volunteers from two different age groups: younger age group between 18–25 years and older age group which was more than 50 years. Histological analysis reveals the following: Monocyte quantity is similar in both S-PRF and A-PRF. Neutrophils are comparatively higher in the above 50 age group. Lymphocytes (S-PRF) more in older than younger age groups. Cells more distributed in A-PRF and concentrated in S-PRF. Loose fibrin in S-PRF and dense fibrin in A-PRF. Although it was found that the older age group could prove to have better properties in PRF, the results are inconclusive due to the limited sample size. Further research is required to enable optimal scaffold formation to be tailored for specific clinical applications.