抗体依赖性细胞介导的细胞毒性
抗体
细胞毒性
生物
分子生物学
免疫学
细胞培养
单克隆抗体
CD16
抗原
体外
生物化学
遗传学
CD3型
CD8型
作者
Takuya Mizuno,Yoshiho Takeda,Toshihiro Tsukui,Masaya Igase
标识
DOI:10.1016/j.vetimm.2021.110315
摘要
Antibody drugs are one of the most important therapeutic modalities in cancer treatment. Antibody drugs for canine cytotoxicity have been developed, and the most important way to evaluate the function of antibody drugs in vitro is to measure the antibody-dependent cellular cytotoxicity (ADCC) activity. However, a good method for measuring the ADCC activity of antibody drugs for dogs has not yet been developed. This study generated a cell line of NK-92 cells, a human natural killer cell line, transfected with canine CD16 and γ-chain as effector cells. A20 cells expressing canine CD20 were used as target cells, and a system for ADCC activity was established using an anti-dog CD20 antibody. The assay generated very consistent results. This is an applicable method for measuring canine ADCC activity and can be used for various antigen-antibody combinations to develop canine antibody drugs with cytotoxic activity in the future.
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