电泳迁移率测定
核糖核酸
电泳
荧光素
化学
RNA结合蛋白
分子生物学
计算生物学
生物
生物化学
基因表达
荧光
基因
物理
量子力学
作者
Shachinthaka D. Dissanayaka Mudiyanselage,Ying Wang
出处
期刊:Methods in molecular biology
日期:2021-11-30
卷期号:: 133-140
标识
DOI:10.1007/978-1-0716-1464-8_12
摘要
RNA-protein complexes are functionally important in biology. Electrophoretic mobility shift assays (EMSA) have been widely used to study the molecular basis of protein-RNA interactions. Previous methods for EMSA mostly relied on radioactive RNA substrates, raising health and environmental concerns. Here, we describe a method based on fluorescein-labeled RNA for EMSA. In addition, we simplified the protocol to efficiently purify RNA-binding proteins from bacterial expression systems.
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