琥珀酰化
赖氨酸
发病机制
人体皮肤
纤维化
多糖
增生性瘢痕
化学
分子生物学
生物化学
生物
医学
免疫学
病理
细胞外基质
氨基酸
遗传学
蛋白多糖
作者
Ke Qiu,Ye Tian,Chunyan Guo,Ougen Liu,Yao‐Cheng Shi,Dewu Liu,Tao Luo
标识
DOI:10.1016/j.jprot.2024.105155
摘要
Lysine succinylation (Ksucc) is a recently identified posttranslational modification that is involved in many diseases. This study examined the role of Ksucc in the pathogenesis of hypertrophic scar (HS). The presence of Ksucc in human skin was measured by immunoblotting. Ksucc occurs in many skin proteins ranging from 25 to 250 kDa, and higher levels of Ksucc are found in HS skin than in normal skin. An immunoaffinity approach coupled with LC-MS/MS was used to characterize the first succinylome of human skin, and 159 Ksucc sites in 79 proteins were identified. Among these, there were 38 increased succinylated sites in 29 proteins but no decreased succinylated sites in HS compared with normal skin. A parallel reaction monitoring assay was performed to validate the results of the succinylome and showed that the levels of Ksucc in decorin and collagens, which are involved in the pathogenesis of HS, were increased in HS than in normal skin. In addition, increasing the level of Ksucc enhanced cell proliferation and upregulated the expression of fibrosis markers (α-SMA, COL1, and COL3) in human skin fibroblasts. Our results provide global insights into the functional role of Ksucc in hypertrophic scarring.
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