Biotechnological Production of Microbial Oils from Carbon Dioxide Using Microalgae and Oleaginous Yeasts in an Integrated Process

Bioprocess engineering optimization enabled the production of Microchloropsis salina biomass in open thin-layer cascade photobioreactors with complete carbon dioxide fixation. Biomass separation, homogenization and enzymatic hydrolysis allowed a partial saccharification of the microalgae biomass. Utilizing a membrane bioreactor with total cell retention, after phosphate depletion, the production of yeast oil in Cutaneotrichosporon oleaginosus was possible despite the dilute sugar hydrolysate.