刺
干扰素基因刺激剂
核酸
癌症免疫疗法
树枝状大分子
小RNA
干扰素
化学
免疫疗法
DNA
细胞生物学
先天免疫系统
生物
癌症
基因
生物化学
受体
免疫学
物理
遗传学
热力学
作者
Rong Wu,Xue Yang,Jiayin Zhan,Zhiyuan Feng,Jun‐Jie Zhu,Jing Jing Zhang
标识
DOI:10.1002/adtp.202300088
摘要
Abstract The cGAS‐STING (cyclic GMP‐AMP synthase/stimulator of interferon genes) pathway is emerging as a promising target for cancer immunotherapy. However, developing specific and effective strategies for activating the cGAS‐STING pathway in tumors is still challenging. Here, a microRNA‐21 (miR‐21)‐responsive nucleic acid system, as a STING signal amplifier, is designed based on the branched catalytic hairpin assembly (bCHA). The effects of three types of dsDNA structures (linear dsDNA, Y scaffold dsDNA, and dsDNA dendrimer) on cGAS‐STING activation are systematically studied. This study demonstrates that dsDNA dendrimer can induce the most effective liquid–liquid phase separation in a miR‐21‐dependent manner, allowing the controllable activation of cGAS‐STING in both cancer cells and dendritic cells. Given the programmable nature of nucleic acid structure, this study will enable a readily accessible platform for integrating with immunotherapeutic strategies while opening new avenues for controllable, tumor‐specific activation of STING agonists.
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