First cultivation of Agaricus flocculosipes and a novel Thai strain of A. subrufescens

Agaricus flocculosipes and A. subrufescens are edible species that belong to section Arvenses of the genus Agaricus.Agaricus subrufescens (almond mushroom) is known to produce bioactive compounds with medicinal properties, such as anti-cancer and anti-tumor activity and fruiting bodies are also edible and nutritious.Agaricus subrufescens is presently cultivated in Brazil, China, Japan, Taiwan and some European countries for use as foods and nutraceuticals.Agaricus flocculosipes is a newly described species currently known only from Thailand, Mayotte Island and China.Species of Agaricus have high potential for cultivation as many species are edible and have medicinal properties.Herein we report the first cultivation of A. flocculosipes and a Thai strain of A. subrufescens.The strains were isolated from wild sporocarps and subcultured on compost extract agar medium.Standard compost based on wheat straw and horse manure was used as the cultivation substrate.A comparative study of the cultivation of one strain of A. flocculosipes and two strains of A. subrufescens from France and Thailand was carried out with four replicates trays.The mushroom spawn was inoculated into the compost and incubated at 25 °C.The mycelia fully covered and grew throughout the media after incubation for two weeks.A casing layer made up of sand, peat and lime (1:1:1) was applied.Fruiting phase was performed at 25 °C and 95% humidity.The first primordia of the French and Thai strains of A. subrufescens occurred after the casing layer was added at days 12 and 24, respectively.Yields were obtained from both strains of A. subrufescens.The yield of the Thai strain (85.90 ± 27.06 g kg -1 ) was lower than that of the French strain (178.56 ± 68.28 g kg -1 ) in the first production cycle lasting 34 days.The first primordia of A. flocculosipes formed at day 32 after casing, and the strain produced only a few fruit bodies (1.04 ± 0.95 g kg -1 ).Further research is needed to develop suitable agriculture wastes and regimes for growing these mushrooms and for increasing production yields so that these strains and species can be industrialized.