赫拉
细胞凋亡
MCF-7型
细胞周期
细胞周期检查点
程序性细胞死亡
DNA断裂
细胞生物学
生物
半胱氨酸蛋白酶
癌细胞
细胞
癌症研究
化学
癌症
生物化学
遗传学
人体乳房
作者
Shumin Liu,Shiyi Ou,Huihua Huang
出处
期刊:Journal of Zhejiang University-science B
[Springer Nature]
日期:2017-02-01
卷期号:18 (2): 89-98
被引量:42
标识
DOI:10.1631/jzus.b1600022
摘要
In order to study the molecular mechanisms of green tea polyphenols (GTPs) in treatment or prevention of breast cancer, the cytotoxic effects of GTPs on five human cell lines (MCF-7, A549, Hela, PC3, and HepG2 cells) were determined and the antitumor mechanisms of GTPs in MCF-7 cells were analyzed. The results showed that GTPs exhibited a broad spectrum of inhibition against the detected cancer cell lines, particularly the MCF-7 cells. Studies on the mechanisms revealed that the main modes of cell death induced by GTPs were cell cycle arrest and mitochondrial-mediated apoptosis. Flow cytometric analysis showed that GTPs mediated cell cycle arrest at both G1/M and G2/M transitions. GTP dose dependently led to apoptosis of MCF-7 cells via the mitochondrial pathways, as evidenced by induction of chromatin condensation, reduction of mitochondrial membrane potential (ΔΨm), improvement in the generation of reactive oxygen species (ROS), induction of DNA fragmentation, and activations of caspase-3 and caspase-9 in the present paper.
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