Improving PHA production in a SBR of coupling PHA-storing microorganism enrichment and PHA accumulation by feed-on-demand control

With volatile fatty acids as substrates, the typical polyhydroxyalkanoates (PHAs) production by mixed culture always includes two steps: PHA-storing culture enrichment via aerobic dynamic feeding strategy and PHA accumulation under nutrient-limited condition. To simplify the PHA-production steps, the enrichment and accumulation step were coupled in a SBR. At start-up period, to investigate the effect of settling selection, one acetate-fed SBR was operated by settling selection-double growth limitation (SS-DGL) strategy, while the other was operated by DGL strategy. The results showed that the stable operation in SBR1 was obtained at about 21, 12 days faster than SBR2, implying the settling selection accelerated the start-up process. After omitting the settling selection under the stable operation, the SBR1 was run above 15 days. The results showed that the performance was not substantial altered. Therefore, the settling selection affected the start-up process but not the stable operation. At operational period, based on the sharp decreasing of oxygen uptake rate (OUR), the poly-β-hydroxybutyrate (PHB) content was improved 13%, from 70 to 83% by feed-on-demand control-double growth limitation (FD-DGL). And the harvested volumetric productivity was 5.0 gPHB/L/day, almost 1-folder improvement. That was to say, the PHB production in a SBR of coupling the enrichment and accumulation step was improved by feed-on-demand control. Meanwhile, the FD experiment can keep steady running for 10 SRTs. Therefore, the SS-DGL/FD-DGL strategy was a promising method for PHA production.