Resistance to RNA interference by plant‐derived double‐stranded RNAs but not plant‐derived short interfering RNAs in Helicoverpa armigera

棉铃虫 生物 RNA干扰 RNA沉默 小干扰RNA 转基因作物 核糖核酸 基因沉默 转基因 植物 基因 生殖器鳞翅目 遗传学
作者
Jinqiu Fu,Shijing Xu,Huan Lu,Fanchi Li,Shengchun Li,Ling Chang,David G. Heckel,Ralph Bock,Jiang Zhang
出处
期刊:Plant Cell and Environment [Wiley]
卷期号:45 (6): 1930-1941 被引量:25
标识
DOI:10.1111/pce.14314
摘要

Abstract Plant‐mediated RNA interference (RNAi) has emerged as a promising technology for pest control through expression of double‐stranded RNAs (dsRNAs) targeted against essential insect genes. However, little is known about the underlying molecular mechanisms and whether long dsRNA or short interfering RNAs (siRNAs) are the effective triggers of the RNAi response. Here we generated transplastomic and nuclear transgenic tobacco plants expressing dsRNA against the Helicoverpa armigera ATPaseH gene. We showed that expression of long dsRNA of HaATPaseH was at least three orders of magnitude higher in transplastomic plants than in transgenic plants. HaATPaseH ‐derived siRNAs are absent from transplastomic plants, while they are abundant in transgenic plants. Feeding transgenic plants to H. armigera larvae reduced gene expression of HaATPaseH and delayed growth. Surprisingly, no effect of transplastomic plants on insect growth was observed, despite efficient dsRNA expression in plastids. Furthermore, we found that dsRNA ingested by H. armigera feeding on transplastomic plants was rapidly degraded in the intestinal fluid. In contrast, siRNAs are relatively stable in the digestive system. These results suggest that plant‐derived siRNAs may be more effective triggers of RNAi in Lepidoptera than dsRNAs, which will aid the optimization of the strategies for plant‐mediated RNAi to pest control.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研乞丐完成签到,获得积分10
刚刚
1秒前
搜集达人应助shaodan采纳,获得100
2秒前
3秒前
oboy应助RP-H采纳,获得10
3秒前
ypz完成签到,获得积分10
3秒前
博修发布了新的文献求助30
4秒前
miawei完成签到,获得积分10
4秒前
晨曦完成签到 ,获得积分10
4秒前
Micheallee完成签到,获得积分10
4秒前
向前完成签到,获得积分10
5秒前
111发布了新的文献求助50
6秒前
刘志斌完成签到,获得积分10
6秒前
corner发布了新的文献求助10
6秒前
轮海完成签到,获得积分10
6秒前
7秒前
童翰完成签到,获得积分10
8秒前
不孤独的发卡关注了科研通微信公众号
8秒前
谢魏楠发布了新的文献求助10
9秒前
博士后完成签到 ,获得积分10
11秒前
13秒前
研友_8WO978完成签到,获得积分10
13秒前
暖冬22完成签到,获得积分10
14秒前
缓慢含烟发布了新的文献求助10
14秒前
奥特波顿完成签到,获得积分10
14秒前
肉松发布了新的文献求助10
14秒前
hehe完成签到,获得积分20
15秒前
完美芹发布了新的文献求助10
15秒前
17秒前
奥特波顿发布了新的文献求助20
17秒前
18秒前
舒展完成签到,获得积分10
19秒前
晨曦完成签到,获得积分10
19秒前
Joekaws发布了新的文献求助30
19秒前
飞仔123发布了新的文献求助10
20秒前
SXYYXS完成签到,获得积分10
21秒前
21秒前
烟花应助Megan采纳,获得30
22秒前
夕沫完成签到,获得积分10
23秒前
23秒前
高分求助中
Les Mantodea de Guyane Insecta, Polyneoptera 2500
Mobilization, center-periphery structures and nation-building 600
Technologies supporting mass customization of apparel: A pilot project 450
China—Art—Modernity: A Critical Introduction to Chinese Visual Expression from the Beginning of the Twentieth Century to the Present Day 430
Tip60 complex regulates eggshell formation and oviposition in the white-backed planthopper, providing effective targets for pest control 400
A Field Guide to the Amphibians and Reptiles of Madagascar - Frank Glaw and Miguel Vences - 3rd Edition 400
China Gadabouts: New Frontiers of Humanitarian Nursing, 1941–51 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3793270
求助须知:如何正确求助?哪些是违规求助? 3337996
关于积分的说明 10288138
捐赠科研通 3054577
什么是DOI,文献DOI怎么找? 1676045
邀请新用户注册赠送积分活动 804058
科研通“疑难数据库(出版商)”最低求助积分说明 761724