微分脉冲伏安法
纳米棒
检出限
生物传感器
材料科学
石墨烯
DNA
介电谱
电极
电化学
循环伏安法
杂交探针
纳米技术
分析化学(期刊)
化学
色谱法
生物化学
物理化学
作者
Huayu Huang,Wanqiao Bai,Chunyan Dong,Rui Guo,Zhihua Liu
标识
DOI:10.1016/j.bios.2015.01.039
摘要
An ultrasensitive electrochemical DNA biosensor for human papillomavirus (HPV) detection was developed by electrochemical impedance spectroscopy and differential pulse voltammetry. A capture probe was immobilized on a glassy carbon electrode modified with graphene/Au nanorod/polythionine (G/Au NR/PT). Two auxiliary probes were designed and used to long-range self-assemble DNA nanostructure. The target DNA can connect DNA structure to the capture probe on the electrode surface. [Ru(phen)3]2+ was selected as a redox indicator for amplifying electrochemical signal significantly. Enhanced sensitivity was obtained through combining the excellent electric conductivity of G/Au NR/PT architecture and the long-range self-assembly DNA nanostructure with the multi-signal amplification. The DNA biosensor displayed excellent performance for HPV DNA detection over the range from 1.0×10–13 to 1.0×10–10 mol/L with a detection limit of 4.03×10–14 mol/L. Furthermore, the proposed method can also be used for the detection of HPV DNA in human serum samples and provides a potential application of DNA detection in clinic research.
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