化学
色谱法
重复性
蛋白酶
固相微萃取
消化(炼金术)
蛋白水解酶
检出限
蛋白酶K
蛋白酵素
萃取(化学)
丝氨酸蛋白酶
生物化学
酶
气相色谱-质谱法
质谱法
作者
Ka-Fai Poon,Paul K.S. Lam,Michael H.W. Lam
标识
DOI:10.1016/s0003-2670(99)00447-x
摘要
A proteolytic digestion — direct immersion solid phase microextraction (SPME) method has been developed for the determination of polynuclear aromatic hydrocarbons in human blood serum. Non-specific serine protease, Proteinase K , was employed to remove the fouling of the SPME stationary phase by the lipoprotein in blood serum during extraction. Optimization of protease concentration and duration of the proteolytic digestion as well as SPME sampling have been performed. Proteinase K concentration of 190 μg protease/ml of serum and digestion time of 60 min at 25°C were found to be the optimum conditions for the determination. PAH recoveries of the direct immersion SPME method ranged from 81.1 to 98.5%. The relative repeatability of the method was found to be 5.6% ( n = 10). Detection limits for the 16 prioritized PAHs ranged from 2.7 to 30.4 ppb.
科研通智能强力驱动
Strongly Powered by AbleSci AI