The Pilocarpine Model of Epilepsy in Mice

Summary: Purpose : To characterize the acute and chronic behavioral, electrographic and histologic effects of sustained seizures induced by pilocarpine in mice. Methods : After status epilepticus, the surviving animals were continuously monitored for 24 h/day for 120 days. The brains were processed by using neo‐Timm and Nissl stains. Results : The first spontaneous seizures occurred between 4 and 42 days after status epilepticus. The mean “seizure‐silent period” lasted for 14.4 ± 11.9 days. During the chronic phase, recurrent spontaneous seizures were observed 1–5 times per animal per week and were associated with sprouting in the supragranular layer of the dentate gyrus. Conclusions : Structural brain damage promoted by pilocar‐pine‐induced status epilepticus may underlie or be associated with recurrent spontaneous seizures in mice.