费斯特共振能量转移
生物传感器
量子点
胶体金
寡核苷酸
荧光
石墨烯
化学
纳米颗粒
材料科学
纳米技术
DNA
生物化学
量子力学
物理
作者
Jingyu Shi,Chun-Yu Chan,Yukting Pang,Weiwei Ye,Feng Tian,Jing Lyu,Yu Zhang,Mo Yang
标识
DOI:10.1016/j.bios.2014.09.059
摘要
In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET biosensor.
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