过氧化物酶
辣根过氧化物酶
材料科学
检出限
催化作用
催化效率
纳米技术
纳米尺度
酶
组合化学
化学
化学工程
色谱法
生物化学
工程类
作者
Xiaohu Xia,Jingtuo Zhang,Ning Lü,Moon J. Kim,Kushal Ghale,Ye Xu,Erin McKenzie,Jiabin Liu,Haihang Ye
出处
期刊:ACS Nano
[American Chemical Society]
日期:2015-09-03
卷期号:9 (10): 9994-10004
被引量:245
标识
DOI:10.1021/acsnano.5b03525
摘要
Peroxidase mimics with dimensions on the nanoscale have received great interest as emerging artificial enzymes for biomedicine and environmental protection. While a variety of peroxidase mimics have been actively developed recently, limited progress has been made toward improving their catalytic efficiency. In this study, we report a type of highly efficient peroxidase mimic that was engineered by depositing Ir atoms as ultrathin skins (a few atomic layers) on Pd nanocubes (i.e., Pd-Ir cubes). The Pd-Ir cubes exhibited significantly enhanced efficiency, with catalytic constants more than 20- and 400-fold higher than those of the initial Pd cubes and horseradish peroxidase (HRP), respectively. As a proof-of-concept demonstration, the Pd-Ir cubes were applied to the colorimetric enzyme-linked immunosorbent assay (ELISA) of human prostate surface antigen (PSA) with a detection limit of 0.67 pg/mL, which is ∼110-fold lower than that of the conventional HRP-based ELISA using the same set of antibodies and the same procedure.
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