Objective:To discuss the clinical application of frozen section in thyroid operation so as to raise the rate of correct diagnosis. Methods: A retrospective review was made for 356 frozen sections and paraffin sections. Results: The frozen section diagnosis showed out 309 cases with benign lesion , 36 cases with malignant tumor and there were 11delayed diagnosis ;in the control , the paraffin section showed 314 cases with benign lesion and 42 cases with malignant tumor ; There were 5 false-negative diagnosis dy frozen section (1.4%) and conformed dinagnosis rate of frozen section reached 98.5%. Conclusion: Correct diagnosing rate of frozen section in thyroid may be raised by increasing the section quality and improving the method.