Analysis of Rumen Bacterial Diversity in Jinjiang Cattle Using MiSeq Sequencing Technology

The present study was designed to reveal the rumen bacterial diversity in Jinjiang cattle using MiSeq high-flux sequencing technology. Ruminal liquid samples were obtained from three Jinjiang cattles( bull)weighted( 400 ± 20) kg with permanent rumen cannulas. Total DNA was extracted and the 16 S rDNA V4 to V5 of bacteria was sequenced by M i Seq sequencing technology. The abundance,distribution and α-diversity index of species were analyzed. The results showed that a total of 56 763 valid 16 S rDNA sequences and 946 operational taxonomic unit( OTU) across 3 samples were obtained. The richness indexes,Chao and Ace indexes,of ruminal bacterial were 836 and 841,respectively; and alpha diversity indexes,Shannon and Simpson indexes were 4.96 and 0.021 5,respectively. Fifteen phyla,23 classes,26 families and 44 genera were identified via taxonomic summary. The dominant phyla among them were Bacteroidetes( 64. 37%),Firmicutes( 21.20%) and Proteobacteria( 7.59%). The most abundant classes were Bacteroidia( 52.88%),Clostridia( 17.03%),and Prevotellaceae( 36.26%). Ruminococcaceae( 9.33%) and Lachnospiraceae( 4.89%) were the predominant family. At genus level,the high abundance of genera were Prevotella( 28.97%),Paraprevotella( 3.08%),Rikenella( 2.28%),Clostridium Ⅳ( 1.77%),Succiniclastium( 1.62%) and Ruminococcus( 1.53%). In conclusion,the diversity of rumen bacterial community in Jinjiang cattle is low. Bacteroidetes is the predominant phylum and Firmicutes is the second most abundant phylum. Prevolla is the most abundant genus in the rumen bacterial community.