Enzymatic Characteristics of 3-Acetyl Deoxynivalenol Oxidase by Devosia sp. DDS-1

【Objective】 The objective of this study is to investigate the enzymatic characteristics of 3-acetyl deoxynivalenol oxidase(3-AC-DON oxidase),understand the degradation characteristics of 3-acetyl deoxynivalenol by Devosia sp.DDS-1,and realize the application of the enzyme.【Method】After being cultured in LG medium,the strain DDS-1 was centrifuged and washed by PBS,and then broken by ultrasonic treatment.The crude enzyme of DDS-1 was obtained by ammonium sulfate precipitation.The enzymatic activity of 3-AC-DON oxidase was then detected in different enzyme reaction systems.【Result】 The optimal 3-AC-DON oxidase production conditions for DDS-1 were 25℃,pH 7.0 and 36 hours in LG medium.The optimum reaction conditions for the 3-AC-DON oxidase in DDS-1 were 35℃,pH 7.0.The enzymatic activity could be maintained above 80% from 20 to 40℃ and in the range of pH 5.0 to 9.0.Most metal ions at a concentration from 0.2 to 2 mmol.L-1could promote the enzymatic activity.Ethylenediamine tetraacetic acid(EDTA) inhibited the enzyme activity on the contrary.The crude enzyme produced by DDS-1 could degrade DON,3-AC-DON in wheat.【Conclusion】The enzymatic characteristics of 3-AC-DON oxidase produced by Devosia sp.DDS-1 was studied in this paper.The results showed that Devosia sp.DDS-1 can produce 3-AC-DON oxidase with good temperature and pH stability,and the enzyme requires metal ions as cofactor.