Development and immunolocalization of stratum intermedium in secretion stage of ameloblasts

Objective To study expression of mineralization - related signaling molecules in stratum intermedium and ameloblasts,to understand the role of stratum intermedium and their interaction in the secretion stage of ameloblasts.Methods BALB/c postnatal mice were applied to study the development of stratum intermedium during the secretion of enamel.PV two steps immunohistochemical method was used to localize the distribution of TNSALP、E -cad、OC、BSP、OPN、BGN、DCN and FM in the enemal organ of day 7 and day 13 old mice.Results TNSALP、E - cad、OC、BSP、OPN、DMP1 and FM were positively expressed in stratum intermedium and ameloblasts.The expression of TNSALP and E - cad in stratum intermedium was earlier than that in ameloblasts,and a spatio - temporal expression pattern could be observed during enamel formation. BSP、OPN、DMP1 and FM were positively expressed in stratum intermedium and ameloblasts in late secretion stage of ameloblasts, but negatively in early stage.Conclusion Stratum intermedium is essential in the secretion stage of ameloblasts, meanwhile,the expression of mineralization - related signaling molecules in stratum intermedium and ameloblasts plays an important regulating role during the formation of enamel.