The expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells

Objective To study the expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells(BTMCs).Methods Reanimating the pEGFP-C1 strain,pEGFP-C1 was extracted and identified. The pEGFP-C1 was embedded in lipofectamine and transfected into BTMCs.The fluorescence intensity of BTMCs after being transfected with the EGFP gene was visualized by fluorescence microscope.Results pEGFP-C1 could be readily transfected into BTMCs.After being transfected,BTMCs with the EGFP gene displayed an effective EGFP expression.Conclusion An EGFP expressing plasmid was used to transfect BTMCs.Transfected cells show a stable EGFP expression in vitro.The study of BTMCs at the genetic level will benefit from this labeling technique.