The results of located experiment on Yellow fluvo aguic soil for 19 years showed that the content of soil organic matter decreased by 1.54 g/kg under no fertilization; Application of chemical fertilizer could maintain the level of soil organic matter; Applying organic materials (yard manure) could increase organic matter by 9.24~ 10.61 g/kg. Compared with CK, N, P, K and manure treatments reduced slightly the oxidation stability of soil organic matter, the opposite was true to the treatments of N and N, P. Under the combined application of manure and chemical fertilizers, organics increased, and the oxidation stability of soil organic matter decreased obviously. Compared with 5 year after start of experiment, the soil humus and humic acid/fulvic acid increased obviously under organic manure application after 19 years. Without fertilization or only chimical fertilizer could not improve the quatity of soil humus.