纤维二糖
化学
色谱法
高效液相色谱法
纤维素
牙髓(牙)
溶解矿浆
水解
色谱检测器
酶水解
纤维素酶
有机化学
医学
病理
作者
Paul B. Filson,Benjamin Dawson-Andoh
标识
DOI:10.1016/j.biortech.2008.12.067
摘要
High-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector was used to quantitatively determine glucose and cellobiose in hydrolyzates from the production of cellulose nanofillers from modified lignocellulosic materials. Prevail Carbohydrate ES 5 micron column proved more suitable for achieving the chromatographic separation of the model pulp hydrolyzate into its constituent sugars than the YMC-Pack Polyamine column. Linear calibration curves for the various sugars in the mixtures were developed. Glucose and cellobiose were clearly detectable in pulp hydrolyzates obtained from enzyme-mediated hydrolysis of recycled pulp, pine and hardwood dissolving pulps. Finally, the amount of glucose in the pulp hydrolyzates was generally higher than cellobiose.
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