树枝状大分子
脂质体
转染
基因传递
赫拉
核酸
细胞毒性
阳离子聚合
共轭体系
遗传增强
化学
生物物理学
组合化学
纳米技术
材料科学
细胞
生物化学
生物
基因
载体(分子生物学)
体外
高分子化学
有机化学
聚合物
重组DNA
作者
Xinyu Wang,Naimin Shao,Qiang Zhang,Yiyun Cheng
摘要
Cationic dendrimers are promising tools in the introduction of nucleic acids into mammalian cells, however, present dendrimer-based gene vectors have modest transfection efficacy and significant toxicity. Here, we synthesized triphenylphosphonium (TPP) conjugated dendrimers and used them as polymeric gene vectors. TPP-conjugated dendrimers can effectively target mitochondria and showed efficient transfection efficacy on HeLa and COS-7 cells with low cytotoxicity on the transfected cells. These materials achieved much higher efficacy than the commercial transfection reagent SuperFect and comparable efficacy with Lipofectamine 2000. This study provides a new insight into the design of efficient and biocompatible gene vectors using dendrimers as scaffolds.
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