氯化胆碱
深共晶溶剂
水解
共晶体系
溶剂
纳米晶
核化学
脂肪酶
甲壳素
对甲苯磺酸
化学
热稳定性
生物催化
化学工程
材料科学
有机化学
壳聚糖
酶
催化作用
离子液体
纳米技术
合金
工程类
作者
Shi‐Lin Cao,Wei-Ming Gu,Wei-Dong Ou-Yang,De-Chu Chen,Yang Bao-yan,Lin-Hao Lai,Yi-Da Wu,Yujia Liu,Jie Zhu,Wanjuan Chen,Zuoqi Gai,Xue-Dan Hou,Yongzheng Ma,Yan-Xia An
标识
DOI:10.1016/j.carbpol.2019.02.092
摘要
Chitin nanocrystal (ChiNC) was fabricated based on p-toluenesulfonic acid -choline chloride deep eutectic solvent treatment. The obtained ChiNC was about 12–44 nm in width and 206–399 nm in length. The crystalline structure and the functional groups of ChiNC were maintained during the preparation process. Moreover, porcine pancreas lipase (PPL) was successfully immobilized onto the ChiNC to form the immobilized PPL ([email protected]). The resulting [email protected] has enzyme loading and activity recovery of 35.6 mg/g and 82.5%, respectively. The thermal stability, pH and temperature adaptabilities of [email protected] was improved, comparing with free PPL. The demonstrated DES treatment process was efficient for ChiNC preparation and the as-prepared ChiNC exhibited great potentials in biocatalysis and biomedical field.
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