生物膜
细菌
抗生素
共价键
嫁接
化学
细菌生长
微生物学
抗菌活性
孵化
金黄色葡萄球菌
核化学
材料科学
有机化学
生物
生物化学
遗传学
聚合物
作者
Ming Li,Haiqing Wang,Xumin Chen,Shengnan Jin,Wei Chen,Yongchun Meng,Yi Liu,Yishun Guo,Wenya Jiang,Xu Xu,Bailiang Wang
标识
DOI:10.1016/j.cej.2019.122973
摘要
Pathogenic bacteria can easily adhere to implanted biomaterials, and this is followed by rapid propagation and biofilm formation. In intravenous prophylactic administration and general topical administration based on physical or non-covalent bonding of antibiotics it is difficult to prevent low concentration release and excessive release, which lead to the development of drug-resistant bacteria. In this work, gentamicin sulfate (GS) was grafted onto aldylated sodium alginate (al-ALG) through dynamic chemical covalent bonding via a Schiff base reaction between aldehyde and amino groups. Antibiotic-loaded sodium alginate was coated on biomaterials by self-assembly with positively charged poly(ethyleneimine) (PEI) to form multilayer films. The ALG-GS/PEI multilayer films showed high antibiotic-loading capacities (223 μg/cm2) through dynamic chemical bonding. The release of GS gave bacterial infection self-defensive features. GS release was much faster at pH 5.5 than at pH 7.4. In vitro antibacterial tests, namely bacterial live/dead staining, zone of bacterial inhibition, and shake-flask culture methods showed efficient bactericidal activity with Staphylococcus aureus and Escherichia coli as model bacteria. ALG-GS/PEI multilayer films killed more than 99.99% of bacteria after 24 h incubation. These antibiotic-loaded multilayer films with long-term, self-defensive, smart bacterial infection and biofilm inhibition properties were almost totally resistant to biofilm development after 72 h incubation. The advantages of chemically grafting antibiotics are reflected in the maintenance of inhibition zone size after storage in a simulated body fluid environment. Dynamic chemical bonding of antibiotics in multilayer films has promising potential applications in biomaterial surface modification.
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