G蛋白偶联受体
受体
加压素
荧光素酶
化学
生物物理学
细胞生物学
逮捕
生物发光
加压素受体
G蛋白偶联受体激酶
能量转移
接受者
生物化学
生物
物理
敌手
内分泌学
转染
基因
分子物理学
凝聚态物理
作者
Edin Muratspahić,Jasmin Gattringer,Christian W. Gruber
出处
期刊:Springer eBooks
[Springer Nature]
日期:2022-01-01
卷期号:: 221-229
标识
DOI:10.1007/978-1-0716-1759-5_13
摘要
Bioluminescence resonance energy transfer (BRET) is a cutting-edge biophysical technique used for exploring G protein-coupled receptor (GPCR) pharmacology. BRET relies on the nonradiative energy transfer from a luciferase energy donor to an acceptor fluorophore after oxidation of a luciferase substrate. This energy transfer occurs only if the donor and acceptor are within close proximity. Over the past few years, BRET has been successfully applied to study GPCR oligomerization as well as interactions of receptors with G proteins, G protein-coupled receptor kinases (GRKs), or β-arrestins. Herein, we describe how BRET can be applied to study signaling at the oxytocin receptor (OTR) and vasopressin receptors, thereby enabling the identification of (biased) ligands and molecular probes for investigating receptor functionality.
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