Microstructure quantification is becoming an important ingredient for predicting material behavior. Here, we present a methodology to quantify 2-phase (α + β) basketweave Ti-6Al-4 V microstructures printed by a wire feed directed Energy Deposition (DED) process. The method focuses on automated quantification of features, such as α lamella thickness and volume fractions of both (α + β) phases, to ensure repeatability and to enable comparison across a wide array of images for subsequent analysis using pre-defined open access image processing libraries in the Python Language. A stereological correction was made for α-lath spacing based on the work of Collins et al. [1], while also assuming area fraction (in 2D images) as equivalent to volume fraction of (α + β) phases. Further, the results were compared with a commercial software package that uses a similar methodology. The methodology is expected to be generally applicable to lamellar microstructures and to other microstructure types via adaptation of the methodology for the features in question.