Cloning a blast resistance gene by chromosome walking

Rice Genetics CollectionRice Genetics III, pp. 669-674 (2008) No AccessCloning a blast resistance gene by chromosome walkingK. S. Wu, C. Martinez, Z. Lentini, J. Tohme, F. G. Chumley, P. A. Scolnik, and B. ValentK. S. WuDuPont Central Research and Development, P.O. Box 80402, Wilmington, DE 19880-0402, USA, C. MartinezCentro Intemacional de Agricultura Tropical, Cali, Colombia, USA, Z. LentiniCentro Intemacional de Agricultura Tropical, Cali, Colombia, USA, J. TohmeCentro Intemacional de Agricultura Tropical, Cali, Colombia, USA, F. G. ChumleyDuPont Agricultural Products, Stine-Haskell Research Center, Newark, DE 19714, USA, P. A. ScolnikDuPont Central Research and Development, P.O. Box 80402, Wilmington, DE 19880-0402, USA, and B. ValentDuPont Central Research and Development, P.O. Box 80402, Wilmington, DE 19880-0402, USAhttps://doi.org/10.1142/9789812814289_0082Cited by:2 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail Abstract: We are progressing toward cloning a blast resistance gene using a map-based cloning strategy. Bulk segregant analysis was used to identify random amplified polymorphic DNA (RAPD) markers tightly linked to the Pi62(t) gene in a doubled haploid population. Screening 1,440 RAPD primers on pooled DNAs from resistant and susceptible plants identified 22 polymorphic bands. Eighteen markers turned out to be linked to the Pi62(t) gene, and three showed no recombination with Pi62(t) in 120 progeny. To facilitate fine-structure genetic and physical mapping, the RAPD markers have been converted into restriction fragment length polymorphisms (RFLPs) and sequence-tagged sites. Five of the Pi62(t)-linked markers have been mapped to the Cornell rice RFLP genetic map, and all are linked to RG869, an RFLP marker that is linked to the blast resistance gene Pi4(t) on chromosome 12. An F2 population consisting of 700 individuals is being used for high-resolution genetic mapping. A bacterial artificial chromosome (BAC) library, consisting of 20,160 independent clones with an average insert size of 110 kb, has been constructed. The total content of the library is equivalent to five haploid genomes of rice. BAC clones have been identified using a single-copy probe, SP7C3, a marker that cosegregates with the Pi62(t) gene. A physical map encompassing Pi62(t) is being constructed. FiguresReferencesRelatedDetailsCited By 2Recent Insights in Rice Blast Disease ResistanceSusheel Kumar Sharma, Devender Sharma, Ram Prasnna Meena, Manoj Kumar Yadav and Rajashekara Hosahatti et al.22 April 2021Quantitative and Qualitative Influence of Inoculation Methods on In Planta Growth of Rice Blast FungusRomain Berruyer, Stéphane Poussier, Prasanna Kankanala, Gloria Mosquera and Barbara Valent1 Apr 2006 | Phytopathology®, Vol. 96, No. 4 Rice Genetics IIIMetrics History PDF download