Changes in expression of NLRP3 inflammasome components and oxidative parameters of mice subjected to high-fat diet and rosa mosqueta oil supplementation

Rosa mosqueta oil (RM) is high in alpha-linolenic acid (ALA) and tocopherols (α-, γ-), which have anti-inflammatory, antioxidant and insulin sensitizing properties, thus preventing the high-fat diet (HFD)-induced damage in mice. To evaluate changes in NLRP3 inflammasome components expression and oxidative parameters in HFD-fed mice supplemented with RM. C57BJ/6 J mice (n=9/group) were fed for 12 weeks and divided into: (i) control diet (CD, 20% proteins, 70% carbohydrates, 10% lipids); (ii) CD+RM (0.01 mL/g bodyweight/day); (iii) HFD (20% protein, 20% carbohydrate, 60% lipids); (iv) HFD+RM. Oxidative stress (carbonylated proteins, MDA content and Nrf2 levels) and NLRP3 inflammasome components (NLRP3, ASC, Caspase-1, IL-1beta) expression in liver and visceral adipose tissue were determined. Results: HFD+RM group showed significantly decreased (two-way ANOVA, bonferroni test, P <0.05) liver and adipose tissue NLRP3 inflammasome expression, along with decreased oxidative stress compared to the HFD group. Dietary RM supplementation decreases NLRP3 inflammasome expression and oxidative stress. This data could be associated with the prevention of metabolic syndrome. FONDECYT 1140547