清脆的
大肠杆菌
质粒
基因敲除
基因
生物
MCR-1型
Cas9
遗传学
微生物学
计算生物学
肠杆菌科
作者
Lichang Sun,Yong Chen,Lili Zhang,Maoda Pang,Qiao-Yan Zhang,Yan Zhou,Hongduo Bao,Ran Wang
出处
期刊:Journal of Microbiology and Biotechnology
[Journal of Microbiology and Biotechnology]
日期:2017-07-28
卷期号:27 (7): 1276-1280
被引量:10
标识
DOI:10.4014/jmb.1611.11021
摘要
The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.
科研通智能强力驱动
Strongly Powered by AbleSci AI