化学
单细胞分析
色谱法
质谱法
吞吐量
存水弯(水管)
细胞
蛋白质组学
栏(排版)
生物化学
基因
工程类
环境工程
电信
结构工程
无线
计算机科学
连接(主束)
作者
Simion Kreimer,Aleksandra Binek,Blandine Chazarin,Jae Hyung Cho,Ali Haghani,Alexandre Hutton,Eduardo Marbán,Mitra Mastali,Jesse G. Meyer,Thássio Mesquita,Yang Song,Jennifer E. Van Eyk,Sarah J. Parker
标识
DOI:10.1021/acs.analchem.3c00213
摘要
Identification and proteomic characterization of rare cell types within complex organ-derived cell mixtures is best accomplished by label-free quantitative mass spectrometry. High throughput is required to rapidly survey hundreds to thousands of individual cells to adequately represent rare populations. Here we present parallelized nanoflow dual-trap single-column liquid chromatography (nanoDTSC) operating at 15 min of total run time per cell with peptides quantified over 11.5 min using standard commercial components, thus offering an accessible and efficient LC solution to analyze 96 single cells per day. At this throughput, nanoDTSC quantified over 1000 proteins in individual cardiomyocytes and heterogeneous populations of single cells from the aorta.
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