Wheat Germ Agglutinin‐Modified “Three‐in‐One” Multifunctional Probe Driven Broad‐Spectrum and Flexible Immunochromatographic Diagnosis of viruses With High Sensitivity

Abstract The conventional lateral flow assay (LFA) fails to the demands for the accurate screening of viruses as a result of its low sensitivity of colorimetric signal output and poor universality limited by antibody pairs. Here, a magnetically assisted dual‐signal output LFA platform is developed for the ultrasensitive, universal, and flexible detection of viruses. A “three‐in‐one” multifunctional probe (MAuDQD) is prepared using a 180 nm Fe 3 O 4 core to load numerous Au nanoparticles (NPs) and two layers of QDs, which can substantially improve the sensitivity of LFA through coupling with the effects of magnetic enrichment and colorimetric/fluorescent enhancement. Wheat germ agglutinin (WGA)‐modified MAuDQD attained the broad‐spectrum capture viral membrane proteins and the colorimetric/fluorescent dual‐mode detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and monkeypox virus (MPXV) on the LFA strip. In the colorimetric mode, the target viruses detected directly, with the visual sensitivity reaching 0.1–0.5 ng mL −1 and the fluorescent mode supported quantitative analysis of SARS‐CoV‐2/MPXV with limits of detection decreasing to pg mL −1 level. Practicability of the MAuDQD@WGA‐LFA is verified through the detection of 33 real clinical samples, showing the proposed assay has a great potential to become a sensitive, accurate, and universal tool for on‐site monitoring of viral infections.