Abstract 3094: Traditional Chinese Medicine Buyang Huanwu Decoction Regulates The Platelet Gpvi And Its Downstream Protein Signaling Pathways,inhibiting Thrombosis And Preventing Cardiovascular Disease.

Background: Coronary heart disease Qi-Deficiency and Blood Stasis Syndrome will lead to the body of platelets disorders, blood viscosity, conducive to the formation of thrombosis, Buyang Huanwu Decoction (BYHWD) is a classic Chinese medicine treatment of Qi-Deficiency and Blood Stasis Syndrome. Platelet glycoprotein VI (GPVI) plays a crucial role in collagen-induced activation and aggregation of platelets. GPVI has become the focus of new approaches to antithrombotic therapy. Methods: After 6 weeks of irregular sleep deprivation in rats with syndrome of deficiency of qi and blood stasis formed, the coronary heart disease model was established by ligation of the left anterior descending coronary artery in rats. The dosage of 1 mL/100 g was given by intragastric administration for 14 days after operation. Results: The purpose of this study was to investigate the effects of BYHWD on platelet granule secretion and platelet function in vitro. We found that BYHWD can significantly reduce the platelet aggregation induced by ADP and collagen, significantly reduce the median expression of CD62p and the ratio of activated platelets, and reduce the concentration of fluorescent calcium ions in platelets. Under the electron microscope observation found that under the action of BYHWD, the ultrastructure of rat platelets pseudopodia reduced, aggregation reduced, rat coronary inner wall platelets, thrombus mass adhesion reduced, and the inner wall was smoother. In addition, BYHWD can regulate GPVI and its downstream proteins. Compared with the coronary heart disease group with syndrome of blood stasis, BYHWD can significantly reduce the expression of GPVI. Significantly reduced the expression of Syk, CALDAG-GEFI, significantly reduced the expression of IP 3 and significantly reduced the degree of PLCγ 2 of rats. Decreased the degranulation reaction of the rat blood, significantly decreased the level of TXA 2 , 5-HT in the rat blood, and significantly decreased the content of the rat blood COX-1 in the rat serum. Conclusion: Therefore, BYHWD can regulate GPVI and its downstream protein signaling pathways, reduce platelet over-activation, degranulation reaction, thereby inhibiting thrombosis, protecting heart function and preventing cardiovascular disease.