Quasi-solid electrochemiluminescence immunosensor constructed with gel electrolyte for the detection of CEA

In this paper, we report a novel quasi-solid electrochemiluminescence (ECL) immunosensor platform for the detection of tumor marker carcinoembryonic antigen (CEA). Referring to the conventional three-electrode system of electrochemistry, a gel alternative solution was introduced, and a quasi-solid solvent-free scheme was designed. The use of gels brings several advantages. Firstly, the introduction of gels also provides a new platform for the specific recognition of antigens and antibodies. Secondly, gels can be mass-manufactured like ELISA plate, which reduces the cost of testing and thus provides a reference scheme for large-scale sensor applications. Thirdly, a new device was designed to accommodate the improved sensors. In addition, a simple one-step hydrothermal method was used to synthesize AuNPs/MnO2/TiO2 materials. Utilizing its excellent catalytic activity for CO-reaction reagents H₂C₂O₄, it promotes the production of CO2–• and thus enhances the ECL of Ru(bpy)32+. Thus, this biosensor provides a novel and efficient strategy for CEA detection. The gel system sensor also provides a general model for the detection of other biomarkers and provides new applications for biochemical analysis and clinical diagnosis.