H3K4me3
胚泡
生物
内细胞团
染色质
组蛋白
染色质重塑
重编程
二价染色质
遗传学
细胞生物学
胚胎干细胞
表观遗传学
心理压抑
胚胎发生
基因
基因表达
发起人
作者
Chuan Zhou,Michelle M. Halstead,Amélie Bonnet-Garnier,Richard M. Schultz,Pablo J. Ross
标识
DOI:10.15252/embr.202255726
摘要
Abstract How histone modifications regulate changes in gene expression during preimplantation development in any species remains poorly understood. Using CUT&Tag to overcome limiting amounts of biological material, we profiled two activating (H3K4me3 and H3K27ac) and two repressive (H3K9me3 and H3K27me3) marks in bovine oocytes, 2‐, 4‐, and 8‐cell embryos, morula, blastocysts, inner cell mass, and trophectoderm. In oocytes, broad bivalent domains mark developmental genes, and prior to embryonic genome activation (EGA), H3K9me3 and H3K27me3 co‐occupy gene bodies, suggesting a global mechanism for transcription repression. During EGA, chromatin accessibility is established before canonical H3K4me3 and H3K27ac signatures. Embryonic transcription is required for this remodeling, indicating that maternally provided products alone are insufficient for reprogramming. Last, H3K27me3 plays a major role in restriction of cellular potency, as blastocyst lineages are defined by differential polycomb repression and transcription factor activity. Notably, inferred regulators of EGA and blastocyst formation strongly resemble those described in humans, as opposed to mice. These similarities suggest that cattle are a better model than rodents to investigate the molecular basis of human preimplantation development.
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