人参
医学
微泡
体内
外体
败血症
药理学
小RNA
细胞生物学
免疫学
生物化学
病理
化学
生物
基因
替代医学
生物技术
作者
Chunhua Ma,Kun Liu,Fei Wang,Xiaochun Fei,Chi‐Chien Niu,Tao Li,Liangming Liu
标识
DOI:10.1097/js9.0000000000000789
摘要
The purpose of this study was to prepare neutrophil membrane engineered Panax ginseng root derived exosomes (N-exo) and investigate the effects of N-exo miRNA 182-5p (N-exo-miRNA 182-5p) on acute lung injury (ALI) in sepsis.Panax ginseng root derived exosomes were separated by differential centrifugation. Neutrophil membrane engineering was performed on exo to obtain N-exo. miRNA182-5p was transmitted into N-exo by electroporation technology to obtain N-exo-miRNA 182-5p. LPS was used to establish an in vivo and in vitro model of acute lung injury (ALI) in sepsis to evaluate the anti-inflammatory effect of N-exo-miRNA 182-5p.The results of transmission electron microscope (TEM) showed that exo was a double-layer membrane structure like a saucer. Nanoparticle tracking analysis (NTA) particle size analysis showed that the average particle size of exo was 129.7 nm. Further, compared with exo, the level of miRNA182-5p was significantly increased in N-exo. The experimental results showed that N-exo-miRNA 182-5p significantly improved ALI via target regulation of NOX4/Drp-1/NLRP3 signal pathway in vivo and in vitro.In conclusion, this study prepared a novel engineered exosome (N-exo) and N-exo-miRNA 182-5p significantly improved ALI in sepsis via target regulation of NOX4/Drp-1/NLRP3 signal pathway, providing new ideas and methods for treatment of ALI in sepsis.
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