探地雷达
内分泌学
内科学
安普克
化学
蛋白激酶A
AMP活化蛋白激酶
糖原合酶
神经元
生物
雌激素受体
细胞生物学
糖原
激酶
医学
神经科学
乳腺癌
癌症
作者
Khaggeswar Bheemanapally,Karen P. Briski
出处
期刊:Neuroendocrinology
[S. Karger AG]
日期:2023-09-12
卷期号:114 (1): 25-41
摘要
The ventromedial hypothalamic nucleus (VMN) is an estrogen receptor (ER)-rich structure that regulates glucostasis. The role of nuclear, but not membrane G-protein-coupled ER-1 (GPER) in that function has been studied. Gene silencing and laser-catapult-microdissection/immunoblot tools were used to examine whether GPER regulates transmitter and energy sensor function in dorsomedial (VMNdm) and/or ventrolateral (VMNvl) VMN counter-regulatory nitrergic and -aminobutyric acid (GABA) neurons. Intra-VMN GPER siRNA administration to euglycemic animals did not affect VMNdm or -vl nitrergic neuron neuron nitric oxide synthase (nNOS), but up-regulated (VMNdm) or lacked influence on (VMNvl) GABA nerve cell glutamate decarboxylase65/67 (GAD) protein. Insulin-induced hypoglycemia (IIH) caused GPER knockdown-reversible augmentation of nNOS, 5'-AMP-activated protein kinase (AMPK), and phosphoAMPK proteins in VMNvl nitrergic neurons. IIH had dissimilar effects on VMNvl (unchanged) versus VMNdm (increased) GABAergic neuron GAD levels, yet GPER knockdown affected these profiles. GPER siRNA prevented hypoglycemic up-regulation of VMNvl and -dm GABA neuron AMPK without altering pAMPK expression. Outcomes infer that GPER exerts differential control of VMNdm versus -vl GABA transmission during glucostasis, and is required for hypoglycemic up-regulated nitrergic (VMNdm and -vl) and GABA (VMNdm) signaling. Glycogen metabolism is reported to regulate VMN nNOS and GAD proteins. Data show that GPER limits VMNvl glycogen phosphorylase (GP) protein expression and glycogen buildup during euglycemia, but mediates hypoglycemic augmentation of VMNvl GP protein and glycogen content; VMNdm glycogen mass is refractory to GPER control. GPER regulation of VMNvl glycogen metabolism infers that this receptor may govern local counter-regulatory transmission in part by astrocyte metabolic coupling.
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