基底膜
纤维连接蛋白
藤黄蛋白C
Tenascin公司
基质(化学分析)
细胞生物学
CD8型
免疫系统
间质细胞
生物
病理
细胞外基质
化学
免疫学
癌症研究
医学
色谱法
作者
Charlotte M. Fonta,Thomas Loustau,Cheng-Bei Li,Suchithra Poilil Surendran,Uwe Hansen,Devadarssen Murdamoothoo,Mario C. Benn,Inés Velázquez-Quesada,Raphael Carapito,Gertraud Orend,Viola Vogel
标识
DOI:10.1016/j.matbio.2023.01.002
摘要
Tracks rich in matrix and cells, as described in several cancer types, have immunosuppressive functions and separate tumor nests and stroma, yet their origin is unknown. Immunostainings of cryosections from mouse breast tumors show that these tracks are bordered by an endothelial-like basement membrane, filled with fibers of collagen adjacent to tenascin-C (TNC) and low-tension fibronectin (Fn) fibers. While present in early-stage tumors and maturing with time, tracks still form under TNC KO conditions, however, host (not tumor cell)-derived TNC is important for track maturation. Tumor infiltrating leukocytes (mostly M2 macrophages and CD8+ T cells) are retained in tracks of early-stage tumors. Following track maturation, retained tumor infiltrating leukocyte (TIL) numbers get reduced and more CD8+ TIL enter the tumor nests in the absence of TNC. As these tracks are enriched with platelets and fibrinogen and have a demarcating endothelial-like basement membrane often adjacent to endothelial cells, this suggests a role of blood vessels in the formation of these tracks. The Fn fiber tension probe FnBPA5 colocalizes with TNC and immune cells in the tracks and shows decreased binding in tracks lacking TNC. Consequently, FnBPA5 can serve as probe for tumor matrix tracks that have immune suppressive properties.
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