Objective: To determine the priority list of key protected, rare and endangered plants in Southeastern Guizhou in order to comprehensively grasp the status quo of key protected and rare plants in southeastern Guizhou. Methods: by adopting key lines and quadrat survey method of combining the field investigation, the use of endangered coefficient, coefficient of genetic value coefficient, habitat type, protection grade coefficient and value coefficient calculated species priority protection value, focus on the protection of qiandongnan prefecture, rare and endangered plant level and priority protection level. Results: There were 33 families, 56 genera and 98 species of national key protected plants and rare and endangered plants in Southeast Guizhou province. Among them, 73 species belonging to 42 genera and 24 families are under state protection, 17 species belonging to 14 genera and 10 families are under key protection in Guizhou Province, 1 species belonging to 1 family, 1 genus and 1 endangered species belong to "Chinese Plant Red Data Book", and 7 species belonging to 4 genera and 4 families are endemic to southeast Guizhou province. 5 species (5.10%) were critically endangered (CR). 23 species (23.47%) were endangered (EN). According to the evaluation of priority protection grade, 8 species were identified as grade i priority protection, accounting for 8.17%. There were 20 species of ii priority protection, accounting for 20.41%. Conclusion: A total of 28 species were critically endangered and endangered, accounting for 28.57% of the total species. There are 28 species, accounting for 28.57%, which are classified as i and ii priority conservation, indicating that there are a large number of rare and endangered plants in Qiandongnan Prefecture, and the conservation work is serious and needs to be strengthened.