Electrochemiluminescence Mechanisms and Bioanalysis Based on Multishape Gold Nanoparticles and Visualized ATT-Au NCs

O nanoparticles (NPs) as quenching probes to precisely induce RET, leading to the ECL signal being switched "off". This dual enhancement and quenching strategy achieved a high signal-to-noise ratio, facilitating the sensitive detection of microRNA-21 (miRNA-21) with a linear range of 1 fM-100 nM and a low detection limit of 0.28 fM. This work not only extends our understanding of the SPC effect and the application of the RET mechanism in ECL, providing a theoretical foundation for further advancements in the ECL field, but also highlights its considerable potential for applications in biomedical research and clinical diagnostics.