转导(生物物理学)
转染
生物
遗传增强
嵌合抗原受体
转基因生物
细胞培养
T细胞
受体
分子生物学
T细胞受体
细胞
细胞生物学
癌症研究
基因
免疫学
免疫系统
遗传学
生物化学
作者
Hadas Weinstein-Marom,Dayana Blokon-Kogan,Maya Levi-Mann,Chaja Katzman,Shira Shalev,Masha Zaitsev,Michal J. Besser,Ronnie Shapira‐Frommer,Gideon Gross,Orit Itzhaki,Lior Nissim
出处
期刊:Methods in molecular biology
日期:2023-12-10
卷期号:: 167-186
标识
DOI:10.1007/978-1-0716-3593-3_13
摘要
Genetic modification of tumor-infiltrating lymphocytes (TILs) or circulating T cells has become an important avenue in cancer therapy. Here we describe a comprehensive method for establishing and expanding TIL cultures and genetically modifying them with a gene of interest (GOI) via retroviral transduction or mRNA transfection. The method includes all the important steps starting with TIL extraction from tumors through to the maintenance of the genetically modified TILs. The protocol includes instructions for retroviral transduction and mRNA transfection of circulating T cells or T-cell lines. The GOIs most commonly introduced into the target cells are chimeric antigen receptors (CARs); genetic adjuvants, such as membrane-bound interleukins; and antitumor T-cell receptors (TCRs).
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